The wistar bloodline has contributed more strains of rats than any other line. Although both rat strains wistar and sd are nearly equally used for the induction and the exploration of the metabolic syndrome features, some seem say that sd rats are less prone for spontenous. Purified lignans were intravenously or orally administered to each male wistar rat. Garcialopez p1, perezurizar j, ibarra a, grijalva i, madrazo i, floresmurrieta f, castanedahernandez g, guizarsahagun g. This demonstration can be used to provide students with an overall conceptual understanding of how to measure renal function. In addition, the red cell life span of the f344 rat was shown to be comparable with other rat strains. Wistar is a world leader in earlystage discovery science in the areas of cancer, immunology and infectious disease. Europe asia and pacific austria baltic states belgium canada croatia denmark finland france germany hungary iceland india ireland israel italy japan luxembourg netherlands norway poland portugal singapore south korea. Depending on the parameters of your experiments, cells from wistar rats, like wistar rat splenocytes, may offer experimental advantages. The metabolism and toxicity of furosemide in the wistar.
By classical definition, a critical size defect is the smallest size tissue defect that will not completely heal over the natural lifetime of an animal. A new genetically obesehyperglycemic rat wistar fatty. They are also used in immunology as models for infectious diseases and autoimmune responses. Determination of median lethal dose of triazophos with dmso in wistar rats article pdf available in asian journal of pharmaceutical and clinical research 74. Li 1college of life science, hohhot, inner mongolia, china 2key laboratory of national education ministry for mammalian reproductive biology and biotechnology, inner mongolia university, hohhot, inner mongolia. The laboratory rat, like its wild counterpart, is an extremely intelligent animal, probably more so than the other rodents commonly used for biomedical research. Cloning, sequencing, and polymorphisms of the wistarimamichi rat growth hormone gene using pcrsscp m. The use of animals in scientific research is not recent.
Metabolic syndrome and hypertension resulting from. During experiments, laboratory animals may experience stress, defined as a nonspecific response of the body to external stimuli. Evaluation of bone regeneration using the rat critical. Dawley from a hybrid hooded male and a female wistar rat. Histological, biochemical, antioxidant enzyme status, as well as the related cytokines and. Most are derived from the albino wistar rat, which is still widely used. Biology department, faculty of applied sciences for girls, umm alqura university, mekkah, ksa. Les rats male et femelle ont ete mis dans deux cages separees, une. Wist rat in chronic toxicity studies scientific poster female wistar han outbred rats as a model of obesity when fed high fat diets. This strain is now disseminated by harland nederland.
Toxic effect of lead on kidney function i n rat wistar f. Determination of erythrocyte life span in f344, wistar, and. Wistar, wistar munich, brown norway, wistar kyoto, shr, and shrsp spontaneously hypertensive rat stroke prone. Hematology and clinical chemistry female wistar han outbred rat. Health status of male adult wistar rats from two experimental. The early investigators who were active at the wistar institute included drs henry donaldson, helen dean king and margaret reed lewis. He later established wistar institute in philadelphia that will have a major role in the development of the rat as an important laboratory animal similar to the impact the jackson laboratory had on the development of the laboratory mouse. The results for the wistar and spraguedawley rats were in good agreement with published data for these strains, thus validating the modified 3hdfp method for erythrocyte life span determination. Wistar rats are thus more representative of human population 7. Nutritional studies on rats fed diets formulated from treated. The institute is committed to accelerating research advances from bench to bedside through brilliant science and distinctive approaches to collaboration among scientific investigators and academic and industry partners. Aoues 2 1 departement of biotechnology, facult y of sciences, university of mostaganem 27000, algeria.
Pricing select your region all others all others e. It has not been established if an outbred rat strain wistars and an inbred rat strain lewis rats exhibit the same sensitivity to taa with regard to the development and course of alf mehendale 2005, rahman. Derived from animals from the wistar institute, philadelphia, pennsylvania. A study was conducted to compare health status of male adult wistar rats from two. A wistar substrain that was disseminated to glaxo laboratory uk from the wistar institute in philadelphia in 1933, then to the dutch institution for nutrition amsterdam, the netherlands and maintained by unilever company vlaardingen, the netherlands from 1941. In this study, the tongue of two species of rat is compared and relation of histological structure of them and their diet is investigated. Wistar plural wistars an outbred albino rat used in medical research, having a wide head, long ears, and a tail.
The rat rattus norvegicus is widely used in experimental work, both providing a rodent model for human ocular disease and as a species for toxicologic screening. Sep 09, 2012 their use in scientific research started in the 16 th century, although the development of the laboratory rat as an experimental model really began in 1906 when the wistar institute developed the wistar rat model rattus norvegicus. Gunma university technical consultant, shibayagi co. Twentyfour adult male wistar rats were employed in the study. We tested the hypothesis that moringa oleifera impairs the morphology and functions of the kidney in rats. In summary, the creation and maintenance of the wistar rats as standardized animals can be attributed to the breeding work of helen dean king, coupled with the management and husbandry methods of milton greenman and louise duhring, and with supporting documentation provided by henry donaldson. A strain of albino rat developed at the wistar institute that has spread widely at other institutionsthis has markedly diluted the original strain. Manuel sur le soin et lutilisation des animaux dexperimentation. Comparison between spraguedawley and wistar rats as an experimental model of pharmacokinetic alterations induced by spinal cord injury. We are only dealing in rabbit and rats for laboratory usage. The rat has been used in experiments since before christ and, in the xix century, with the intensification of the research reports, the use of this species increased considerably.
Wistar rat article about wistar rat by the free dictionary. A rat can be lifted by the base of the tail, but this technique should be employed for only short periods, such as to move a rat. Research laboratory animals wistar rats manufacturer from. The laboratory rat handbook of experimental animals. Files are available under licenses specified on their description page. Pharmacological investigation of the wound healing. In the case of bone, an orthotopic defect that will not heal without intervention is termed a critical size defect. Donalson at the wistar instiute, usa, at the begining of 19th century. Wistar rat definition of wistar rat by the free dictionary. Pdf determination of median lethal dose of triazophos. Panel of rat cell lines cls cell lines service part of the cls cell bank table 1. Wistar rat medical definition merriamwebster medical.
The common laboratory rat, rattus norvegicus, has had a colorful history in its progression from feral origins to the research laboratory. The wistar institute is an independent, nonprofit research institution in biomedical science, with special expertise in oncology, immunology, infectious disease and vaccine research. Are sprague dawley rats better than wistar rats for obesity. Wistar rats definition of wistar rats by the free dictionary. Tol, ont ete examinees chez le rat wistar femelle gestante. Evolution, history and domestication of the norway rat. Their use in scientific research started in the 16 th century, although the development of the laboratory rat as an experimental model really began in 1906 when the wistar institute developed the wistar rat model rattus norvegicus.
Comparison between spraguedawley and wistar rats as an. The various published classifications of ovarian tumors in many species require the availa bility of clinical information for their full application. Pdf spermatozoa morphology and characteristics of male. Morphometric and anatomical evaluation of the heart of. The oldest purposebred strain of inbred rats, the pa strain, had been created by helen dean king at the wistar institute in philadelphia by 1909 krinke 2000. Hence in the present study, we used the deninduced hepatic cancer model in wistar rats and evaluated the cancer inhibition effects of the aqueous extract of ajwa dates ade. Characterization of six rat strains rattus norvegicus by. A total of 16 male and female rats rattus norvegicus, of conventional wistar lineage, originating from the central animal facility at the technical unit of the biological sciences and health center ufms were used in this study. Renal biomarkers of male and female wistar rats rattus. Effects of shortterm fasting in male spraguedawley rats ncbi. The animals were randomly distributed into two groups.
To define the nature of the toxic metabolite, we examined the relationship between furosemide metabolism in cd1 mice and wistar rats. Furosemide, a loop diuretic, causes hepatic necrosis in mice. Comparative pharmacokinetics of purified flaxseed and. Therefore we studied whether wistar rats obtained from harlan laboratories ha, n 24 and charles river cr, n 22 had a different outcome in an acute myocardial. The present study was aimed at investigating the wound healing effect of ethanolic extract of cestrum nocturnum l. With their more active behaviour and higher metabolic rate 7, 15, wistar rats are less sensitive towards t2d than spraguedawley rats. Wistar han list of studies wistar han rats species strain sex route vehicle chemical rats wistar han male and female gavage corn oil pentabromodiphenyl ether mixture de71 technical grade rats wistar han male and female gavage corn oil tetrabromobisphenol a rats wistar han male and female gavage water green tea extract rats wistar han.
Pdf in summary, the creation and maintenance of the wistar rats as. The wistar institute is a global leader in biomedical research with special expertise in cancer, immunology, infectious diseases and vaccine development. The similar species rattus norvegicus, the brown rat or wharf rat, has also been carried worldwide by ships in recent centuries. The zucker fatty rat as a genetic model of obesity and hypertension pdf. Moringa oleifera impairs the morphology and functions of. Scientific poster historical background control data in the rcchan. Another popular laboratory rat is the longevans, also known as the hooded rat, as it has darker hair over parts of the head and anterior body. Cpcsea committee for the purpose of control and supervision on experiments on animals headed by a government official from a reputed institute and others from various committees for.
The common domestic rat, which is found worldwide and is a destructive pest of crops and stored food and a carrier of disease. In the era when biology was being defined, he recognized in the rat. Genetically defined strains of laboratory animals, part i, mouse and rat p. Rat mouse default values rat default values subchronic study chronic study weaning bw water intake water factor food intake food factor bw water intake water. Wistar rats synonyms, wistar rats pronunciation, wistar rats translation, english dictionary definition of wistar rats. The originality of the diet we used was the age at. In combination with wky strain, a wistar diabetic fatty rat was developed with increased fatness, glucose intolerance, decreased insulin sensitivity, hepatic insulin resistance, neuropathic. Male wistar rats were anesthetized with nembutal and were intravenously injected with 1 mgkg of fab2 or fab fraction of either monoclonal antibody 5i2, which inhibits the function of rat counterpart of mouse crryp65, or monoclonal antibody 6d1, which inhibits the rat counterpart of cd59. The purpose of this protocol is to demonstrate the principles and techniques for measuring and calculating glomerular filtration rate, urine flow rate, and excretion of sodium and potassium in a rat. Elle a notamment produit des rats spraguedawley knockout, avec les techniques zincfinger nucleases zfn.
All structured data from the file and property namespaces is available under the creative commons cc0 license. Three groups were used for the induction of heterotopic bone, but with different volumes. Wistar rats definition of wistar rats by medical dictionary. Approche quantitative des comportements nocturnes chez le rat wistar. A laboratory rat or lab rat is a rat of the species rattus norvegicus domestica which is bred and. Handling is seen as the mani pulation of an animal by hand for the purposes of removing it from the cage and transferring it to another cage, onto the pan of a balance for weighing. The widespread use of the wistar rats, however, is a function of the ingenuity of milton greenman who. One of the noninbred lines which they maintained was known as the wistar rat. Wistar rats from different suppliers have a different.
While less commonly used for research than mice, rats have served as an important animal model for research in psychology and biomedical science. The norms and organization of the animal facility are in total compliance with. Located in the university city section of philadelphia, wistar was founded in 1892 as americas first nonprofit institution solely focused on biomedical research and training. They are thought to have originated in some parts of asia. Spontaneous ovarian neoplasms of the laboratory rat. Previous evidence suggested hepatotoxicity arises from metabolic bioactivation to a chemically reactive metabolite that binds to hepatic proteins. This is reflected in its behaviour and, when treated properly and sympathetically, its tolerance to handling and restraint. Pdf toxic effect of lead on kidney function in rat wistar.
They are commonly used in many fields of research, including oncology, aging, pharmacology, toxicology, and metabolismnutrition. Located in the university city section of philadelphia, wistar was founded in 1892 as americas first nonprofit institution solely focused on biomedical research. The survey, performed at the 10th generation of backcrossing, showed that wistar fatty rats fafa, a congenic strain of wky, developed obesity and obesityrelated features, such as hyperinsulinemia and hyperlipemia, in the same manner as zucker fatty rats. Wistar rats 220250 g body weight were purchased from the laboratory animal center of heilongjiang university of chinese medicine harbin, china and divided into two groups group a, drug group for dosed rat plasma, n 7, and group b, control group for blank rat plasma, n 7 and kept in a breeding room. Tsh is produced and secreted by basophilic cells called thyrotrophs in the anterior. Rats from this colony were sent all over the world, beginning as early as 1911. The wistar rat is a commonly used strain for experimental animal models. An inbred strain is defined as a strain that has been through at least 20 generations of sibmating or its. The wistar rat is one of the two most routinelyused outbred albino rat strains in biomedical research. Handling is seen as the mani pulation of an animal by hand for the purposes of removing it from the cage and transferring it to another cage, onto the pan of a. Experimental model of heterotopic ossification in wistar rats. Origin and development the wild brown rat rattus norvegicus is thought to have had its original habitat in the temperate areas of what is now the ussr, from the caspian sea to northern china. Wistarkyoto rat how is wistarkyoto rat abbreviated.
A laboratory rat or lab rat is a rat of the species rattus norvegicus domestica which is bred and kept for scientific research. Origin and general characteristics name of cell line cell type organism, ethnicity age gender tissue disease morphology growth properties cls order no. The fagene was transferred from the zucker rat m strain to the wistar kyoto wky rat. Ten animals from each strain were sampled from different matings. Wistar albino rats were divided into five groups each consisting of six animals. Learn how to pronounce wistar and more about the wistar word at. Hematology and clinical chemistry male wistar han outbred rat. Ah 1 rat liver hepatoma cell line rattus norvegicus rat spraguedawley. The rat is either restrained by an assistant or may be anaesthetized by a shortacting narcotic agent and its penis extruded by sliding the prepuce downwards while pressing at the base of the penis figure 24. Recently it was shown that results vary between studies using wistar rats of different suppliers. Wistar rat definition of wistar rat by medical dictionary. Morphometric and anatomical evaluation of the heart of wistar rats. Cloning, sequencing, and polymorphisms of the wistarimamichi.
Wistar rat splenocytes can be used for a wide variety of immunologybased applications. Apparently indigenous to the steppes of asia, the species is thought to have migrated down the norwegian peninsula and arrived in england via ship in the early 18th century donaldson, 1924. While being well studied in spraguedawley rats 712, long term effects of fructose enriched diet are not in wistar rats. The laboratory rat, rattus norvegicus springerlink. Spermatozoa morphology and characteristics of male wistar rats administered with ethanolic extract of lagenaria breviflora roberts. Dawley rats are used in the study of embryonic development, teratology and reproductive toxicology, as. Purpose this standard operating procedure sop describes guidelines for the management of rat breeding colonies. To nederlandsinstituut voor volksfoending in 1933, to unilever, vlaardingen in 1941 and institut centraale proefdierenbedrijf tno in 1958. Wistar rat synonyms, wistar rat pronunciation, wistar rat translation, english dictionary definition of wistar rat.
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